Ok Prof. Nandini, let's see how well you do (just came out of my biochem class a few days ago -- we focused on DNA / RNA / Amino Acids ... so I think returning the tension of studying and passing the class is justified, even though the professor is different 😆):
The following are worth 0.5 points each:
1. In the helix-turn-helix motif of DNA binding proteins, the amino acid side chains of one helix binds to the _______ and the other to the ______.
2. The enzyme that is capable of synthesizing DNA from an RNA template is ______.
3. The DNA binding structure similar to the prokaryotic helix-turn-helix is ________.
4. In E. coli, the signal sequence that properly aligns the initiation codon in the P site is called the ____ sequence.
5. UV light causes damage to DNA by forming _____.
6. The Rho () protein terminates transcription by acting as a ___.
7. _____ causes an increase in transferrin receptor levels and a decrease in ferritin levels.
8. The twofold axis of symmetry of the lac repressor matches the symmetry of the _______.
Total points: __ / 4
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Multiple Choice Questions (1 point each):
1. What are significant features in the development of induced pluripotent stem cells?
A) Only four transcription factors are required to induce pluripotency in fibroblasts.
B) De-differentiated cells have characteristics of embryonic stem cells.
C) Potentially, iPS cells represent a new class of therapeutic agents.
D) All of the above.
E) None of the above
2. Molecules that bind to a receptor and trigger signaling pathways are called
A) antagonists.
B) agonists.
C) IRE-binding proteins
E) None of the above.
3. A chemical commonly used to induce the lac operon in laboratory experiments is
A) lactose.
B) X-Gal.
C) IPTG
D) All of the above.
E) None of the above.
4. Which of the following conditions leads to the lysogenic state?
A) Repressor high, Cro high
B) Repressor low, Cro low
C) Repressor high, Cro low
D) Repressor low, Cro high
E) None of the above
5. In eukaryotes, DNA-binding proteins bind to DNA by which of the following DNA-binding structures?
A) Cys2 His2 zinc-finger domain
B) Homeodomain
C) Closed loops called lariats
D) A and B
E) A, B, and C
6. The actual three-dimensional structure of tRNA is
A) L-shape.
B) cloverleaf. .
C) twisted triple helix.
D) All of the above.
E) None of the above
Total points: / 6
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Short response (2 points each)
1. List the different subunits of E. coli RNA polymerase holoenzyme complex.
(Just list them, no description)
2. What are the minimal requirements for DNA replication?
Total points: / 4
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Bonus Question (1 point, freebie point if answered):
What field of Biochem research are you involved in?
Can you draw the full reaction mechanism of Glycolysis? (yes or no)
++++++++++++++++++++++++++++++++
Actually, you don't have to answer any of those question :P I'm more curious about the Bonus Questions 😆
Edited by shyam09 - 11 years ago
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